Development of different optimized strategies and characterization of a Dened Mixed Consortia (DMC) for enhanced LDPE bacterial biodegradation
Author
Martinez I Quer, Alba
Term
4. term
Education
Publication year
2019
Submitted on
2019-06-30
Pages
85
Abstract
Dette studie undersøger bæredygtige måder at øge polyethylens (PE) biologiske nedbrydelighed. Tre strategier blev afprøvet: tilsætning af en biosurfaktant (MEL), tilsætning af et co-substrat (et ekstra næringsstof til mikroorganismer) og brug af et defineret blandet konsortium (en nøje udvalgt gruppe bakterier). Bakterierne i konsortiet blev isoleret efter en tre måneders berigelse, og der blev udviklet en mikrotiterplade-metode til effektiv screening af mange kombinationer. FTIR-spektroskopi, som kan afsløre kemiske ændringer i materialer, viste en svag oxidation af C=C-dobbeltbindinger—et tegn på biologisk nedbrydning—i prøver behandlet med de mest succesfulde konsortier. Stammernes genomer blev sekventeret for at lede efter relevante enzymer. Gener for laccaser og alkanhydroxylaser blev fundet i nogle isolater, hvilket understøtter deres potentiale for polymeroxidation. Studiet peger på dye-decolourizing peroxidase som et lovende enzym til at nedbryde, eller facilitere nedbrydning af, lavdensitetspolyethylen (LDPE) i konsortier.
This study explores sustainable ways to make polyethylene (PE) more biodegradable. Three strategies were tested: adding a biosurfactant (MEL), adding a co-substrate (an extra food source for microbes), and using a defined mixed consortium (a carefully selected group of bacteria). The bacteria in the consortium were isolated after a three-month enrichment, and a microtiter-plate method was set up to efficiently screen many combinations. FTIR spectroscopy, which detects chemical changes in materials, showed slight oxidation of C=C double bonds—a sign of biodegradation—in samples treated with the most successful consortia. The genomes of the strains were sequenced to look for relevant enzymes. Genes coding for laccases and alkane hydroxylases were found in some isolates, supporting their potential to oxidize polymers. The study highlights dye-decolourizing peroxidase as a promising enzyme for degrading, or facilitating the degradation of, low-density polyethylene (LDPE) within consortia.
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