Development of different optimized strategies and characterization of a Dened Mixed Consortia (DMC) for enhanced LDPE bacterial biodegradation
Author
Term
4. term
Education
Publication year
2019
Submitted on
2019-06-10
Pages
85
Abstract
Different strategies were proposed as enhancers of the inner biodegradability of polyethylene, in a sustainable approach: the supplement of a biosurfactant (MEL) or a co-substrate, and the use of a dened mixed consortia (DMC). Bacteria used for the DMC were isolated from a 3-month enrichment and a microtiter culture methodology was developed for high-throughput screening. FTIR analysis conrmed a weak oxidation in C=C bonds due to biodegradation within the most successful consortia. Furthermore, the genome of the strains was sequenced and a putative study of the presence of different enzymes was carried out. Laccases and Alkane hydroxylases were found within the genome of some of the isolates, which conrmed its putative capacity for polymer oxidation. Dye-decolourizing peroxidase was proposed to be a promising LDPE degrading enzyme or a facilitator enzyme within the consortia.
Keywords
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