A master thesis from Aalborg University
Development of different optimized strategies and characterization of a Dened Mixed Consortia (DMC) for enhanced LDPE bacterial biodegradation
Author(s)
Term
4. term
Education
Publication year
2019
Submitted on
2019-06-10
Pages
85 pages
Abstract
Different strategies were proposed as enhancers of the inner biodegradability of polyethylene, in a sustainable approach: the supplement of a biosurfactant (MEL) or a co-substrate, and the use of a dened mixed consortia (DMC). Bacteria used for the DMC were isolated from a 3-month enrichment and a microtiter culture methodology was developed for high-throughput screening. FTIR analysis conrmed a weak oxidation in C=C bonds due to biodegradation within the most successful consortia. Furthermore, the genome of the strains was sequenced and a putative study of the presence of different enzymes was carried out. Laccases and Alkane hydroxylases were found within the genome of some of the isolates, which conrmed its putative capacity for polymer oxidation. Dye-decolourizing peroxidase was proposed to be a promising LDPE degrading enzyme or a facilitator enzyme within the consortia.
Keywords
Documents
Colophon: This page is part of the AAU Student Projects portal, which is run by Aalborg University. Here, you can find and download publicly available bachelor's theses and master's projects from across the university dating from 2008 onwards. Student projects from before 2008 are available in printed form at Aalborg University Library.
If you have any questions about AAU Student Projects or the research registration, dissemination and analysis at Aalborg University, please feel free to contact the VBN team. You can also find more information in the AAU Student Projects FAQs.