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A master's thesis from Aalborg University
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The Effect of Landfill Leachate on Nitrification Inhibition of Activated Sludge

Author

Term

4. term

Publication year

2025

Pages

63

Abstract

Efterhånden som spildevandet bliver mere komplekst, skal renseteknologier forbedres for at understøtte bæredygtig drift. En nøgleproces er nitrifikation, den biologiske omdannelse af ammoniak til nitrat, som udføres af følsomme mikroorganismer. Giftige stoffer kan hæmme denne proces og forringe udløbskvaliteten. Dette speciale kombinerer et litteraturstudie om nitrifikationshæmning (NI) – herunder kilder, testmetoder og nitrifikanters modstandsdygtighed – med kontrollerede laboratorieforsøg. Vi anvendte en test af ammoniak-omdannelseshastighed til at vurdere, om en prøve af perkolat fra en losseplads (vand, der siver gennem affald) hæmmer nitrifikation. N-allylthiourinstof (ATU), en kendt nitrifikationshæmmer, blev brugt som reference for at bekræfte, at metoden kan påvise hæmning. I vores forsøg forårsagede det undersøgte perkolat ikke målbar NI. På den baggrund anbefaler vi at klassificere og begrænse kilder til spildevand for at beskytte nitrificerende mikroorganismer og sikre en stabil rensningsydelse.

As wastewater becomes more complex, treatment technologies must improve to support sustainable operation. A key step is nitrification, the biological conversion of ammonia to nitrate carried out by sensitive microorganisms. Toxic substances can inhibit this step and harm effluent quality. This thesis combines a literature review on nitrification inhibition (NI)—including sources, test methods, and the resilience of nitrifying microbes—with controlled laboratory experiments. We used an ammonia conversion rate assay to evaluate whether a sample of landfill leachate (water that drains through waste) inhibits nitrification. N-allylthiourea (ATU), a known nitrification inhibitor, served as a reference to confirm that the assay can detect inhibition. In our tests, the examined leachate did not cause measurable NI. Based on these findings, we recommend classifying and restricting wastewater inputs to protect nitrifying microorganisms and maintain stable treatment performance.

[This summary has been rewritten with the help of AI based on the project's original abstract]