Screening of enzymatic cleaning of UF membrane fouled with oat slurry
Author
Hassan, Ismail Mohamed
Term
4. semester
Publication year
2023
Submitted on
2023-06-01
Pages
44
Abstract
Demand for plant-based beverages such as oat drinks is increasing, yet the commonly used decantation step in production is energy-intensive and offers poor separation. Membrane filtration is a promising alternative but suffers from fouling caused by proteins, lipids, and fibers in oat slurry. This project investigated how much pure water flux (PWF) can be recovered by enzymatic cleaning of a PES ultrafiltration membrane in a plate-and-frame configuration after fouling with oat slurry. The study prepared oat slurry, selected suitable enzymes, and established cleaning protocols by systematically varying enzyme type, concentration, temperature, cleaning time, sequence, and the number of cleaning cycles. An alkaline chemical cleaning served as a reference, and cleaning performance was evaluated by flux recovery; surface properties such as hydrophobicity and charge were also examined using water contact angle and zeta potential. The highest flux recovery was achieved by alkaline chemical cleaning (21.65%), whereas an enzymatic sequence using acidic enzymes followed by a basic enzyme reached 14% recovery. These findings provide a baseline for optimizing greener cleaning strategies for membrane filtration in oat drink production, although chemical cleaning delivered the highest flux recovery in this screening.
Efterspørgslen efter plantebaserede drikke, herunder havredrik, er stigende, men den udbredte decantering i produktionen er energikrævende og giver lav separationsgrad. Membranfiltrering er et lovende alternativ, men udfordres af fouling fra bl.a. proteiner, lipider og fibre i havreslurry. Dette projekt undersøgte, hvor meget ren vand-flux (PWF) der kan genvindes ved enzymatisk rengøring af en ultrafiltreringsmembran af PES i en plate-and-frame-konfiguration efter fouling med havreslurry. Opgaven omfattede fremstilling af havreslurry, udvælgelse af egnede enzymer og etablering af rengøringsprocedurer med systematisk variation af enzymtype, koncentration, temperatur, rengøringstid, sekvens og antal rengøringscykler. En kemisk basisk rengøring blev anvendt som reference, og rengøringseffekten blev vurderet ved fluxgendannelse; derudover blev overfladeegenskaber som hydrofobicitet og ladning undersøgt via vandkontaktvinkel og zeta-potentiale. Den højeste fluxgendannelse blev opnået med basisk kemisk rengøring (21,65 %), mens en enzymatisk protokol med sure enzymer efterfulgt af et basisk enzym gav 14 % genvinding. Resultaterne giver et grundlag for at optimere grønne rengøringsstrategier til membranfiltrering i havredriksproduktion, selv om kemisk rengøring i denne screening gav den højeste fluxgendannelse.
[This apstract has been generated with the help of AI directly from the project full text]