Investigation of the Self-assembling Properties of the Peptide KA5 Family
Translated title
Undersøgelse af de selv-organiserende egenskaber af peptidfamilien KA5
Author
Eeg, Ruben Peter
Term
4. term
Education
Publication year
2015
Submitted on
2015-09-15
Pages
79
Abstract
This thesis investigates how the surfactant-like KA5 peptide family self-assembles, focusing on chain-length variants (KA4, KA5, KA6) and the effect of terminal amidation. It situates the work within a bionanotechnology context and reviews the fundamentals of surfactant and peptide self-assembly. The peptides were produced by solid-phase peptide synthesis, purified by HPLC, and subsequently washed with HCl. Self-assembled structures were generated in vitro and imaged by atomic force microscopy; a fluorescence-based Thioflavin-T assay is also described for characterization. In parallel, force-field simulations were used to model aggregation in silico, and computed structures were qualitatively compared with experimental observations. The excerpt indicates that nanostructures were formed and imaged, but does not include detailed results or conclusions; these are presented in later chapters.
Dette speciale undersøger, hvordan den surfaktant-lignende peptidfamilie KA5 selv-organiserer, med fokus på kædelængdevarianter (KA4, KA5, KA6) og betydningen af terminal amidation. Arbejdet sætter studiet ind i en bionanoteknologisk kontekst og gennemgår den grundlæggende teori for selv-organisering af surfaktanter og peptider. Peptiderne blev syntetiseret ved solid-phase peptide synthesis, oprenset med HPLC og efterfølgende vasket med HCl. De dannede strukturer blev fremkaldt ved selv-organisering in vitro og visualiseret med atomkraftmikroskopi; desuden beskrives et fluorescensbaseret Thioflavin-T-assay som karakteriseringsmetode. Parallelle kraftfeltsimuleringer blev udført for at modellere aggregering in silico, og de beregnede strukturer blev kvalitativt sammenholdt med de eksperimentelt observerede. Uddraget angiver, at der blev dannet og afbildet nanostrukturer, men indeholder ikke detaljerede resultater eller konklusioner; disse præsenteres i senere kapitler.
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Keywords
self-assembly ; KA5 ; peptide