A master thesis from Aalborg University
Identification of adipose stem cells subsets by the use of polychromatic flow cytometry
Author(s)
Term
4. term
Publication year
2020
Submitted on
2020-05-29
Pages
32 pages
Abstract
Better understanding of phenotype profiling and functionality of adipose stem cells subsets may significantly improve stem cell treatment. Previous studies identified and sorted out populations with co-expression of four and six markers with diverse expression pattern. Among typical mesenchymal stem cell markers CD73+CD90+CD105+ other markers primarily associated with adipogenesis, osteogenesis, chondrogenesis, wound healing and immodulatory properties were examined. This study aims to identify expression profiles of five and eight co-expressed markers in two panels by the use of polychromatic flow cytometry. Additionally, stem cell were analyzed according to their culturing method- in a regular culture flask or in a bioreactor.
Keywords
Documents
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