Functional analysis of microRNA-target gene interactions related to Intestinal Bowel Disease
Author
Raunbak, Sofie
Term
4. term
Publication year
2019
Submitted on
2019-05-31
Pages
94
Abstract
Inflammatorisk tarmsygdom er en voksende global byrde, og mikroRNAer er lovende regulatorer, men det er udfordrende at kortlægge deres funktionelle mål. Denne afhandling havde til formål at identificere og validere funktionelle mikroRNA–mål-gen-par relevante for IBD ved at klone kandidat-3'UTR-sekvenser fra IL12A, ZEB1, SOCS1 og TRIM32 ind i psiCHECK2-dual-reporterplasmidet og måle luciferase/Renilla-udtryk efter co-transfektion i cellekultur. Der blev observeret statistisk signifikante ændringer i reporteraktiviteten, som indikerer interaktioner mellem miR-21-5p og IL12A, miR-150-5p og ZEB1 samt miR-155-5p og SOCS1. Fundene giver eksperimentel evidens for udvalgte miRNA–mRNA-interaktioner med potentiel relevans for langsigtede behandlingsstrategier mod IBD, men yderligere forskning er nødvendig for at afklare reguleringsmekanismer og sygdomsmæssig betydning.
Inflammatory bowel disease is an increasing global burden, and while microRNAs are promising regulators, mapping their functional targets remains challenging. This thesis aimed to identify and validate functional microRNA–target gene pairs relevant to IBD by cloning candidate 3'UTR sequences from IL12A, ZEB1, SOCS1 and TRIM32 into the psiCHECK2 dual-reporter plasmid and measuring luciferase/Renilla expression after co-transfection in cultured cells. Statistically significant changes in reporter activity indicated interactions between miR-21-5p and IL12A, miR-150-5p and ZEB1, and miR-155-5p and SOCS1. These findings provide experimental support for selected miRNA–mRNA interactions with potential relevance for long-term therapeutic strategies in IBD, while highlighting the need for further studies to clarify regulatory mechanisms and disease impact.
[This summary has been generated with the help of AI directly from the project (PDF)]
Keywords
Documents