A master programme thesis from Aalborg University
Enhancing the Polyphenol profile and Antioxidant properties of Crithmum Maritimum Extract from Cascading Biorefienry by Lactic Acid fermentation
[Styrkning af den Polyphenol og antioxidante egenskaber af Crithmum Maritimum ekstrakt med kaskadende Bioraffinaderi igennem Lactic Acid fermentering]
Author(s)
Term
4. semester
Education
Publication year
2025
Submitted on
2025-06-02
Pages
186 pages
Abstract
Crithmum Maritimum, which is a salt tolerant halophyte beach plant, is chosen to be tested on to see if its possible to enhance its polyphenol and antioxidant profile. This is done to to see if its can be enhanced for biorefiney purposes and to increase its value as a potential bio remedy for soil salinization. Therefore making the plant valuable for the pharmaceu- tical/ nutraceutical industry The enhancement is done by solid fermentation with lactic acid bacteria, due to its probi- otic chracteristics. Different strains and pretreatment methods were used to improve the yield, polyphenol content and antioxidant activity. Soxhlet extractions were used ther- after to extract the contnents of the biomass. Thereafter all the results were applied in an in silico study of extracting polyphenols from the biomass on an industrial scale. Based on the trials, Thermal pretreated Crithmum Maritimum fermented with Lactobacillus Plantarum achieved the best results with an extraction yield of 32.93%, Polyphenol con- tent of 25.18 mg/ml and IC50 of 3.32 mg/ml. In the techno-economical analysis conducted, two cases, one based on former reports and the other on the laboratory experiments, were simulated which one is more feasi- ble. Case 1 with fractionated biomass was in the end not feasible, but Case 2 with whole biomass was feasible. Case 2 can therefore be seen as possible plan for a biorefinery using Crithmum Maritimum as feedstock.
Keywords
Documents
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