An improved and efficient method for the extraction of phycobiliproteins for optimization of phycocyanin productivity in Leptolyngbya sp. QUCCCM 56 under different irradiance and temperature
Author
Fortunati, Filippo
Term
4. term
Education
Publication year
2018
Pages
21
Abstract
Visse cyanobakterier (blågrønbakterier) og rhodofyter (rødalger) indeholder vandopløselige pigmenter, kaldet fykobiliproteiner. Disse pigmenter opfanger farver af lys, som klorofyl kun absorberer svagt, og udvider dermed det lys, der kan omsættes til kemisk energi. For at understøtte fysiologiske studier har vi udviklet en praktisk protokol til at ekstrahere fykobiliproteiner fra tre mikroorganismer: Leptolyngbya sp. QUCCCM 56, Arthrospira platensis og Rhodomonas sp. Protokollen kombinerer forskellige metoder til celleopbrud, valg af ekstraktionsopløsning, mængde biomasse og inkubationstider. For alle testede stammer svarede ekstraheringseffektiviteten til det, der er rapporteret i litteraturen, mens pigmentrenheden var højere end i litteraturen (Arthrospira platensis 100 %, Rhodomonas sp. 288 % og Leptolyngbya sp. 370 %). Med denne protokol undersøgte vi, hvordan lysintensitet (80–1800 µmol·m−2·s−1, en standardenhed for fotosyntetisk lys) og temperatur (20–45 °C) påvirker produktionen af pigmentet fykocyanin i Leptolyngbya sp. QUCCCM 56. Den højeste fykocyanin-produktivitet—0,091 gram pr. liter pr. dag—blev målt ved 300 µmol·m−2·s−1 og 40 °C. Fykocyaninindholdet faldt ved højere lysintensiteter og steg ved højere temperaturer. Biomasseproduktiviteten havde et maksimum ved 300 µmol·m−2·s−1, og højere temperaturer øgede yderligere produktiviteten.
Some cyanobacteria (blue-green bacteria) and rhodophytes (red algae) contain water-soluble pigments called phycobiliproteins. These pigments capture colors of light that chlorophyll absorbs poorly, expanding the light available for converting sunlight into chemical energy. To support physiological studies, we developed a practical protocol to extract phycobiliproteins from three microorganisms: Leptolyngbya sp. QUCCCM 56, Arthrospira platensis, and Rhodomonas sp. The protocol combines different ways to break open cells, choices of extraction solution, biomass amounts, and incubation times. Across all tested strains, extraction efficiency matched published reports, while pigment purity was higher than in the literature (Arthrospira platensis 100%, Rhodomonas sp. 288%, Leptolyngbya sp. 370%). Using this protocol, we examined how light intensity (80–1800 µmol·m−2·s−1, a standard unit of photosynthetic light) and temperature (20–45°C) affect production of the pigment phycocyanin in Leptolyngbya sp. QUCCCM 56. The highest phycocyanin productivity—0.091 grams per liter per day—occurred at 300 µmol·m−2·s−1 and 40°C. Phycocyanin content decreased at higher light intensities and increased at higher temperatures. Biomass productivity peaked at 300 µmol·m−2·s−1, and higher temperatures further increased productivity.
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