A study of human CD11c as a potential receptor for targeted vaccines
Translated title
Studie af humant CD11c som mulig receptor for targeterede vacciner
Author
Søndergaard, Fie Hedemann
Term
4. term
Publication year
2015
Submitted on
2015-05-29
Pages
61
Abstract
Dendritiske celler er centrale immunceller, der viser små dele af mikrober eller kræft (antigener) til andre immunceller, og de undersøges som grundlag for terapeutiske kræftvacciner. Et overfladeprotein kaldet CD11c er et lovende mål til at styre vacciner mod dendritiske celler, men de fleste studier er lavet i mus, og der vides mindre i menneskelige systemer. Dette projekt undersøgte, hvordan CD11c er fordelt blandt blodceller, om to anti-CD11c-antistofkloner udløser modning af dendritiske celler, og om disse antistoffer optages (internaliseres) af dendritiske celler. Vi brugte flowcytometri (en metode, der måler proteiner på tusindvis af enkeltceller) og mikroskopi, herunder konfokalmikroskopi, til at undersøge dette. Vi fandt, at CD11c findes på både dendritiske celler og monocytter i blod. Ingen af antistofklonerne fremkaldte modning af dendritiske celler. Begge antistofkloner blev internaliseret af dendritiske celler med 12,63% og 16,27% optagelse. Resultaterne tydeliggør CD11c-fordelingen og hvordan anti-CD11c-antistoffer interagerer med dendritiske celler i blod.
Dendritic cells are key immune cells that show pieces of microbes or cancer (antigens) to other immune cells and are being explored for therapeutic cancer vaccines. A surface protein called CD11c is a promising target to direct vaccines to dendritic cells, but most studies have been done in mice, and less is known in human systems. This project examined how CD11c is distributed among blood cells, whether two anti-CD11c antibody clones trigger dendritic cell maturation, and whether these antibodies are taken up (internalized) by dendritic cells. We used flow cytometry (a technique that measures proteins on thousands of individual cells) and microscopy, including confocal microscopy, to investigate these questions. We found that CD11c is present on both dendritic cells and monocytes in blood. Neither antibody clone induced dendritic cell maturation. Both antibody clones were internalized by dendritic cells, with 12.63% and 16.27% uptake. These results clarify CD11c distribution and how anti-CD11c antibodies interact with dendritic cells in blood.
[This abstract was generated with the help of AI]
Keywords
Documents