Polymeric drug delivery system

Student thesis: Master thesis (including HD thesis)

  • Camilla Lystlund Andersen
4. term, Nanotechnology, Master (Master Programme)
This Master project addresses the potential application of polyvinylpyrrolidone-based polymer micelles as a drug delivery system. 1 and 6 kDa polymer was used to form either micelles loaded with curcumin as a model hydrophobic drug, or to create empty micelles by applying chloroform as a temporary hydrophobic stabiliser. The physical properties of the synthesised micelles were studied using Nanoparticle Tracking Analysis to determine the size distribution of the micelles, and Atomic Force Microscopy to visualize them. Glioblastoma (U87) and Fibroblast (CRL 2429) cell lines were used for in vitro studies of the application of polymeric micelles in drug delivery, with the main focus being on the mechanisms of drug uptake in the cells as well as cytotoxicity. The cellular uptake of curcumin in micellar- and free form was studied using Optical Fluorescent Microscopy. Cytotoxicity assays were used to determine the mortality of the cell lines, when incubated with different concentrations of micellar and free curcumin, as well as empty micelles, for 24 hours. The studies of endocytosis mechanisms were carried out using different endocytosis inhibitors including dynasore, worthmaninn and sodium azide, followed by either microscopic visualization of the cells or cytometry measurements. Although no final conclusion was reached on the uptake mechanism, it was successfully demonstrated that the micellar form of curcumin drastically enhances the uptake and efficacy of the drug formulation, as compared with the free form.
Publication date3 Feb 2017
Number of pages100
ID: 250648911