Nanophotonics circuits formed by DNA self-assembly

Student thesis: Master thesis (including HD thesis)

  • Steffan Møller Sønderskov
4. term, Nanotechnology, Master (Master Programme)
This thesis investigated the use of DNA origami as the structural element in a nanophotonics circuit consisting of gold nanoparticles placed with high precision. Theoretical predictions on the fluorescence enhancement of the ATTO-647N dye in the gap of gold nanoparticle dimers were made. It was found that the dye was enhanced 385 times when in a gap of 15 nm surrounded by 100 nm particles. Predictions were also made for other gap and particle sizes. The evidence of the correct self-assembly of an 18 hb DNA origami structure was difficult to determine due to the small size, although AFM and agarose electrophoresis both pointed to the formation of an origami structure. Self-assembly of a 42 hb origami structure was found to proceed correctly according to gel electrophoresis while AFM imaging showed well-defined and correctly folded structures. Conjugation of 15 nm gold nanoparticles onto the 42hb structure was found to achieve a high success-rate of approximately 91 % in addition to precise placement.
Publication date15 Sep 2016
Number of pages113
ID: 240407419