The aim of this study was to investigate the motivation for participating in an eSports club and the emphasis on physical activity. A multiple case study was conducted on three eSports clubs supported by Danish Gymnastics and Sports Associations (DGI) in Denmark. 10 eSports club members (all males, aged 10-15 years) participated voluntarily in the study. The participants (n=10) all took part in an interview, which were carried out in the respective eSports clubs in a one-to-one setting. Furthermore, the training was observed to determine the level of physical activity. Self-Determination Theory (SDT) was chosen as the theoretical basis for a semi-structured interview, whereas physical activity was observed using lightly structured passive participant observation. The data were analyzed using interpretative phenomenological analysis (IPA) and discussed in regard to the terms autonomy, competence, and relatedness as well as physical activity and challenges for participation in an eSports club. The study showed that both autonomy, competence and relatedness were present for the members in the eSports clubs, but relatedness seemed to be the most important predictor for the members’ participation. Furthermore, eSports clubs contribute towards health and promote physical activity.