• Jannie Boss Nielsen
Introduction: Iron deficiency is a widespread micro nutritional disorder and affects approximately 2 billion people worldwide. Iron demand increases notably during pregnancy, from 0.8 mg/day in the first trimester to >6 mg/day in the third trimester, thus, making it difficult to maintain an acquired iron level without any iron supplementation. Hippocampus plays an important role in processing information and behavior through a complex interaction with amygdala and hypothalamus. Through oxidative phosphorylation, the high energy requirement from hippocampus is met. Cytochrome c oxidase is an iron-containing terminal enzyme involved in the last step of the oxidative phosphorylation reaction. Thus, making it a quantifiable marker for neuronal metabolic activity. Aim: The aim of this study was to establish a rodent experimental model to investigate how iron deficiency will affect behaviour and neurodevelopment in offspring rats and whether supplementation with iron isomaltoside (Monofor) at two various stages (gestation day 19 and postnatal day five) of development can prevent brain abnormalities. Method: 14 Wistar rats were divided into four groups; Control group (CN), gestation day 19 (G19), postnatal day one (ID) and postnatal day five (P5). G19, ID and P5 received all iron deficient diet and injection of Monofer at the corresponding days. Offsprings were tested for any fear and anxiety-like behavior with open field, elevated zero maze, and light/dark box, where exploration were assessed for 10 min. Areas in hippocampus were stained for cytochrome c oxidase activity and used to support the findings in the behavior tests. Results: In open field, nearly all tests showed CN was significant different compared to all three groups. At day >28 in distanced travelled, G19 showed a significant increase compared to ID. Elevated zero maze revealed a significant increase in number of stretches between G19 and ID. Light/dark box showed that ID and P5 performed better compared to CN and G19. Dot blot assays did not reveal any significant difference between groups, however, there was seen a tendency for CN, G19, and P5 to have an increased cytochrome c oxidase activity compared to ID. Conclusion: Growth and hemoglobin showed that iron supplementation had an effect compared to ID. The behavioral study did not revealed any significant difference between the two supplementation groups compared to ID. However, CN had a better performance in open field compared to ID. There were no significant findings in the biochemical assays
Publication date31 May 2018
Number of pages37
ID: 280182515