Student thesis: Master thesis (including HD thesis)

  • Iva Sakota
Background: Urethral strictures have recurrent nature; therefore, treatment techniques often remain
limited. Silk fibroin (SF) has shown promising results in the repair of urethral strictures, especially in
combination with seeded cells. Still, the best combination of seeded cells on the SF scaffold is yet to
be discovered.
Aim: The aim of this project is to investigate a method for the assessment of SF tissue engineered
sheets aimed for the repair of urethral defects.
Methods: For the fabrication of the scaffolds, four different methods have been applied;
electrospinning, freestanding silk films, freeze drying and stepwise deposition of SF. Afterwards, the
scaffolds were seeded with cells; keratinocytes and fibroblasts. Thin polyester inserts were used as a
control and transepithelial electrical resistance (TEER) was measured. Lastly, immunostaining of cells
was carried out to track cell proliferation.
Results and conclusion:
When it comes to scaffold fabrication, all the methods used in this study, beside free-standing silk
films, had good mechanical properties and thickness and were support cell proliferation. SF showed
to have desirable properties for cell growth, thus no difference between SF coated and the
membranes without coating can be found. Measures of TEER showed inconsistent values over time,
which might be due to detachment of the SF coating from the surface and difficulties while
performing the method. Also, it was found that co-seeding with fibroblasts had effect on the
formation of TJ in keratinocytes only when seeded on SF coated inserts.

Publication date31 May 2018
Number of pages53
ID: 280190267