Twelve Danish athletic sub-elite sprinters (six female and six male) were tested to determine the acute effect of a heavy back squat and sled towing, on 40 meter maximal sprint performance. The study consisted of a repeated measures design with three experimental conditions. During the control (CON) intervention, the participants performed a 30 minutes warm up, then a six minutes rest before performing a 40 meter maximal sprint test. For the squat intervention (SQ), the participants performed a 30 minutes warm up then one set of three repetitions 90% of one repetition maximum (1RM). The participants then had a six minutes rest before performing a 40 meter maximal sprint test. For the sled towing intervention (SL), the participants performed a 30 minutes warm up then a 15 meter maximal sled towing sprint. The participants then had a six minutes rest before performing a 40 meter maximal sprint test. The results showed no significance between the three interventions P=0.146 in the ANAOVA test.