Software Defined Radio (SDR) is a piece of equipment that uses software as a replacement for traditional hardware components. Recently, a new type of SDR, which is a PCI-connected SDR, has been created. These have a very low latency meaning that it should be possible to implement protocols like Bluetooth and WiFi on them, which was not previously possible due to high latency.\\
This project focuses on investigating whether or not it is possible to use Bluetooth Low Energy (BLE) with a PCI-connected SDR to communicate while upholding the time requirements of the BLE protocol. A Proof of Concept (PoC) is created to investigate this and test this hypothesis. The PoC contains one of these new SDRs, and a lot of focus has been put on configuring it for optimal performance in terms of speed. The PoC also uses a third-party library as a foundation to implement the lower layers of BLE's protocol stack. The library is also modified to function with the specific use cases of this PoC. Critical link layer functionality was also implemented to handle different communication scenarios.\\
The results are that the PoC can receive and respond within the required time of the BLE protocol. The PoC offers a functioning lower-layer implementation of BLE along with a foundation on which further BLE functionalities can be implemented. The project also gives recommendations on how to further expand on this PoC.