Better understanding of phenotype profiling and functionality of adipose stem cells subsets may significantly improve stem cell treatment. Previous studies identified and sorted out populations with co-expression of four and six markers with diverse expression pattern. Among typical mesenchymal stem cell markers CD73+CD90+CD105+ other markers primarily associated with adipogenesis, osteogenesis, chondrogenesis, wound healing and immodulatory properties were examined. This study aims to identify expression profiles of five and eight co-expressed markers in two panels by the use of polychromatic flow cytometry. Additionally, stem cell were analyzed according to their culturing method- in a regular culture flask or in a bioreactor.