Development of a new polymericDNA delivery system

Studenteropgave: Speciale (inkl. HD afgangsprojekt)

  • Miquel Santos Llinas
4. semester, Nanobioteknologi (cand.polyt.), Kandidat (Kandidatuddannelse)
Gene therapy is a promising strategy
for curing diseases that have a genetic origin, but so far it has been limited due to the difficulties of delivering DNA to human cells. In this
thesis, a new non-viral DNA delivery system has been created using the
amphiphilic polymer PVP-OD and a
short cationic peptide. Some properties of the new nanocarrier such as the
size, the shape or the safety have bee
studied, as well as its efficiency inducing foreign gene expression in human cells. Although the nanocarrier
seems optimal for gene delivery, high
levels of transfection has not yet been
observed, suggesting that more work
is necessary to optimize this new system. Considering the promise that the
PVP-OD nanocarrier may have, and
given the technical limitations that the
2D transfection experiments have, a
COMSOL model has been created to
aid in the future development of a microfluidic chip that could be used for
testing this DNA delivery system in
conditions that more closely resemble
real humans.
Udgivelsesdato3 jun. 2021
Antal sider113
ID: 413604982