A fungal strain Neurospora sitophila is analysed to utilize Second generation lignocellulosic biomass press cake and brown juice under submerged and solid-state fermentation condition. Three main enzyme FPAase , CMCase and -glucosidase were studied in both conditions. The Solid-state fermentation condition is found most suitable for enzyme activity. Sterile and non-sterile media is also analysed but heavy contamination is observed in non-sterile media. The different dilutions of brown juice with water were evaluated and (5%) Brown juice found supportive for microbial enzyme activity. Two different temperature were also studied and different agricultural residues such as (wheat bran, wheat grains and Oat grains) were also analyzed in SSF condition. Wheat bran (1%) was found inducive for higher enzyme activity. Three different additional nutrients (Jaggery, Urea and Mustard oil) were also supplemented in SSF to find out their role as inducer but there was less enzyme secretion was performed by fungal strain. Finally, after optimizing some conditions a SSF based tray bioreactor was designed in lab for batch operation. A notable increment in enzyme activity was observed.