A master thesis from Aalborg University
Nanophotonics circuits formed by DNA self-assembly
[Nanofotonisk kredsløb skabt ved DNA selv-samling]
Author(s)
Term
4. term
Education
Publication year
2016
Submitted on
2016-09-15
Pages
113 pages
Abstract
I dette speciale blev det undersøgt hvorvidt DNA origami kunne anvendes som struktur-element i et nanofotonisk kredsløb bestående af guld nanopartikler. Teoretiske forudsigelse vedr. fluorescens stigningen af ATTO-647N i en guld nanopartikel dimer hul blev udarbejdet. Stigningen blev fundet til at være i omegnen af 385 for en hul-størrelser på 15 nm omgivet af 100 nm partikler. Forudsigelser blev også udarbejdet for andre hul-størrelser samt partikel størrelser. Bevis for en korrekt selv-samlet 18 hb DNA struktur var udfordrende pga. dens størrelse. Dog pegede både agarose gelelektroforese samt AFM undersøgelser på, at en origami struktur var blevet dannet. Den korrekte selv-samling af 42 hb origami struktur kunne ses ud fra agarose gelelektroforese samtidig viste AFM analyse også veldefineret og korrekte foldede strukturer. Konjugeringen af 15 nm guld nanopartikler til en 42 hb DNA origami struktur blev fundet til at opnå en succesrate på 91 % foruden en præcis placering.
This thesis investigated the use of DNA origami as the structural element in a nanophotonics circuit consisting of gold nanoparticles placed with high precision. Theoretical predictions on the fluorescence enhancement of the ATTO-647N dye in the gap of gold nanoparticle dimers were made. It was found that the dye was enhanced 385 times when in a gap of 15 nm surrounded by 100 nm particles. Predictions were also made for other gap and particle sizes. The evidence of the correct self-assembly of an 18 hb DNA origami structure was difficult to determine due to the small size, although AFM and agarose electrophoresis both pointed to the formation of an origami structure. Self-assembly of a 42 hb origami structure was found to proceed correctly according to gel electrophoresis while AFM imaging showed well-defined and correctly folded structures. Conjugation of 15 nm gold nanoparticles onto the 42hb structure was found to achieve a high success-rate of approximately 91 % in addition to precise placement.
Keywords
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