Multivalent nanoparticle interactions of membranes to design targeting systems for cells
Author
Term
4. term
Education
Publication year
2018
Submitted on
2018-06-08
Pages
117
Abstract
The understanding of how nanoparticles (NPs) specifically bind to receptors in cell membranes is still lacking. In particular, a focus on decorating NPs with specifically binding ligand for target receptors have largely ignored the influence of nonspecific colloidal interactions on targeting affinity. Thus, model systems were established in order to set a basis for interpretation of the effect on the avidity of NPs. Silica NPs were coated with lipid bilayers by liposome fusion, using different composition liposomes that would modulate the interaction potential of the particles. A controlled density of biotin functional groups in the lipid membrane served as a model for specific interactions with avidin proteins. The quality of the coating was estimated by dynamic light scattering and zeta potential. An optimal coating procedure was developed. In addition, supported lipid bilayers and poly(ethylene glycol) surface coatings were self-assembled on silica sensor surfaces with different coverages of avidin proteins to mimic the cell membrane and used to investigate the specific and non-specific interactions with coated silica NPs via Quartz Crystal Microbalance with Dissipation monitoring.
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