Electrospinning of fibre embedded cells as scaffolds for muscle regeneration: A STUDY OF THE SUITABILITY OF ELECTROSPUN FIBRES FOR TISSUE ENGINEERING
Translated title
Electrospinning of fibre embedded cells as scaffolds for muscle regeneration
Author
Peñas Tabernero, Laura
Term
4. term
Education
Publication year
2017
Submitted on
2017-06-10
Pages
71
Abstract
Regenerativ medicin har til formål at reparere eller erstatte beskadiget væv. Selvom vævsteknik allerede har nået nogle kliniske forsøg, er genopbygning af skeletmuskulatur stadig en stor udfordring. Dette projekt undersøger en ny måde at fremstille cellevenlige stilladser på ved hjælp af koaksial elektrospinning, en metode hvor et elektrisk felt spinder to materialer til én kerne-skal-fiber. Metoden kan indkapsle levende celler i fiberkernen og orientere fibrene, så cellevækst styres i én retning, hvilket er vigtigt for muskel. Vi vurderede, om celler kunne overleve (levedygtighed), dele sig (proliferation) og modnes til muskelceller (differentiering) på disse fibre. Flere celletyper blev overvejet, med fokus på myogene C2C12-celler fra mus. Disse celler overlevede elektrospinningprocessen og forblev levedygtige over tid. Fibre med en skal af polycaprolacton (PCL) og en kerne af polyethylenoxid (PEO)/alginat var optimale til fremstilling af det fibrøse stillads. Der blev dog ikke påvist differentiering, så den regenerative effekt skal undersøges nærmere.
Regenerative medicine aims to repair or replace damaged tissues. While tissue engineering has already reached some clinical trials, rebuilding skeletal muscle remains a major challenge. This project explores a new way to make cell-friendly scaffolds using coaxial electrospinning, a method that uses an electric field to spin two materials into a single core-shell fiber. The approach can encapsulate living cells inside the fiber core and align the fibers so cell growth is guided in one direction, which is important for muscle. We assessed whether cells could survive (viability), multiply (proliferation), and mature into muscle cells (differentiation) on these fibers. Several cell types were considered, with a focus on mouse myogenic C2C12 cells. These cells survived the electrospinning process and stayed viable over time. Fibers made with a polycaprolactone (PCL) shell and a polyethylene oxide (PEO)/alginate core were optimal for fabricating the fibrous scaffold. However, we found no evidence of differentiation, so the regenerative potential of this system still needs further study.
[This abstract was generated with the help of AI]
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