A master thesis from Aalborg University
Characterization of 12 kDa PVP-OD Nanocarriers and the Influence of Their Size on the Uptake in Mammalian Cells
Author(s)
Term
4. term
Education
Publication year
2019
Submitted on
2019-06-10
Pages
71 pages
Abstract
Nanocarrier drug delivery systems can greatly improve bioavailability, circulation times and delivery of drugs. Cellular uptake mechanisms of nanocarriers are greatly influenced by their physicochemical properties. In this master thesis, the characteristics of the 12 kDa amphiphilic block copolymer PVP-OD is investigated and the influence of nanocarrier size on the uptake in fibroblasts (CRL 2429) examined. Therefore, nanocarriers with a size of 220 nm (0.2 PDI) and 21 nm (0.244 PDI) were created by sonification or cosolvent evaporation, respectively. Both nanocarriers were taken up by a energy-dependent process, since inhibition led to a decrease of median fluorescent intensity (MFI) of 90 or 97 percent for sonificated and cosolvent evaporated nanocarriers, respectively. Colocalization of the cosolvent evaporated nanocarriers drug with LysoTracker indicated a possible macropinocytosis or clathrin-mediated endocytic uptake. However, further study with different endocytic inhibitors is necessary.
Keywords
Documents
Colophon: This page is part of the AAU Student Projects portal, which is run by Aalborg University. Here, you can find and download publicly available bachelor's theses and master's projects from across the university dating from 2008 onwards. Student projects from before 2008 are available in printed form at Aalborg University Library.
If you have any questions about AAU Student Projects or the research registration, dissemination and analysis at Aalborg University, please feel free to contact the VBN team. You can also find more information in the AAU Student Projects FAQs.